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Research Outcomes


LCF Research Outcomes

01-LCF-55

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Vocational Training and access to decent work for women domestic workers, transgender individuals, and persons with disabilities: Reflections on Gender, labor and equality for social inclusion of disadvantaged groups of Hazara Division

Prof. Dr. Abdul Majid, Department of Management Sciences, University of Haripur

Department of Management Sciences, Hazara University Mansehra

Comsats University Islamabad, Attock Campus

Lack of decent work opportunities for women domestic workers, transgender individuals, and persons with disabilities is creating issues of employment and social inclusion for these socially and economically disadvantaged groups.

The primary objective of this project is to provide vocational trainings to selected disadvantaged groups, of Hazara Division to facilitate their access to decent work.

Prepared comprehensive databases using qualitative and qualitative data collection techniques to identify women domestic workers, transgenders, and persons with disability.

Identified current employment status, socio-economic conditions, and the need for training to adopt decent work roles using surveys, interviews, structured observation, and other data collection tools.

Designed suitable interventions based on their interests, with the assistance of vocational training providers in the social/ private sector.

 

02- LCF-229

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Pre-mature Fruit Drop: A Local Challenge of Apple Growers in Rawalakot, Azad Jammu & Kashmir

PI:
Dr. Noosheen Zahid
Department of Horticulture, Faculty of Agriculture, University of Poonch Rawalakot

Co-PI:
Prof. Dr. Aman Ullah Malik, Institute of Horticultural Sciences, University of Agriculture, Faisalabad

Co-PI:
Dr. Mehdi Maqbool,
Department of Horticulture, Faculty of Agriculture, University of Poonch Rawalakot

To investigate potential causes of pre-mature fruit drop.

To explore influence of different plant growth regulators, micronutrients, pesticides and fungicides on fruit set and fruit drop patterns. And the interactive effect of plant growth regulators, micronutrients, pesticides and fungicides on fruit drop control, fruit ripening and quality of apples at harvest.

To analyse data to increase apple production and increase income of the farmers

Apple orchards from three different locations selected.

Soil analysis indicated deficiency of organic matter and lack in Nitrogen. A few are deficient in Boron and Magnesium.

Fruit and leaf samples collected from all 3 selected locations. Pathological screening showed that different fungal species such as Colletotrichum, Alternaria, Penicillium and Fusarium were responsible for pre-mature fruit drop in apples. Sooty blotch was the most significant disease appeared on apple fruits.

Codling Moth (Cydia pomonella) was recorded as the key pest of apples for this region. More than 55 % damage was recorded by Codling Moth only.

Different plant growth regulators, pesticides, insecticides, soil nutrients and their interactive effect on pre-mature fruit drop is under investigation.

 

03-LCF-151

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Strengthening of Quality education at Gilgit Baltistan by using digital platformsPI:
Dr. Aftab Ahmed Khan, Department of Computer Sciences, Karakorum International University, Gilgit, Gilgit-Baltistan.

City University of Science and Information Technology, Peshawar.

National University of Science and Technology, Islamabad 

To improve affordability, accessibility, and quality of education in Gilgit Baltistan through the digitization of selected schools from different sectors (Private and public).

To Develop an intelligent and customized Learning Management System for education.

 

Compiled a baseline survey report to know the status of digitalization that includes resources, requirements, and challenges in schools and colleges of Gilgit-Baltistan.

Developed websites for project and schools: lcf.kiu.edu.pk

Planned, designed, and developed a customised Learning management system for schools and colleges

 

04-LCF–181

Climate change adaptations for indigenous coal desulfurization using ionic liquids

Lead PI - Prof. Dr. Uzaira Rafique , Dean Fatima Jinnah Women University, Rawalpindi (FJWU).

Co-PI - Dr. Aliya Fazal, Assistant Professor, FJWU.

Research Student - Ms. Sofia Laraib, FJWU

Academic Collaborators - Prof. Dr. Habib Nasir, NIST, Isb.

Sectoral Partners:  Taj Muhammad, AM, Pakistan Mineral Development Corporation;  Mr. Ali Iqtidar,  Deputy Manager, Geo Sindh Engro Coal Mining Company.

To collect indigenous coal samples and process/ handle as per ASTM methods

To synthesize greener solvents for desulfurization of coal samples.

 

 

 

Sectoral collaborators (PMDC and SECMC) helped in provision of 22 coal samples from Sindh and Baluchistan collieries

Processed/ proximated analysis of coal samples per ASTM Standard methods at labs of applied chemistry, FJWU,

Physiochemical characteristics of coal samples including Sulphur content was tested from Hydrocarbon Development Institute of Pakistan.

Imidazolium and ammonium based ionic liquids are synthesized

The synthesized ILs were screened / characterized using Fourier Transform Infrared Spectroscopy for application,

 H-NMR and TGA-DSC characterizations (in progress).

 

05-LCF-10

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Plasma nanoscience-based transition metal
dichalcogenides nanostructured electrode materials for energy storage applications

Department of Physics, Government College University Faisalabad

Principal Investigator:
Dr. Ijaz Ahmad Khan

Co-Principal Investigator:
Dr. Abdur Rasheed
Dr. M Zahir Iqbal

National Collaborators:
Dr. M. Fakhar-e-Alam
Dr. M. Usman
Dr. Uzma Ikhlaq

International Collaborator:
Prof. Dr Lujun Pan

To develop sustainable and efficient simple PNSB-TMDs-NEM for energy storage lithium batteries via LTNPCA by employing PECVD system.

 

To Synthesize hybrid PNSB-TMDs-NEM for energy storage lithium batteries, via LTNPCA by using PECVD system.

 

To evaluate and optimize the electrochemical performance of simple and hybrid PNSB-TMDs-NEM to achieve highest possible electrochemical capacitance, energy density, powder density, cyclic stability, rate capability, charging-discharging capacities, capacity retention and capacity fading.

 

To train Research Associate, M. Phil and PhD students on this technology

 Initial material testing has started but the project is still in progress as it was affected by dollar fluctuation (increased cost) and COVID outbreak.

 

 

06-LCF-14

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Factors Inhibiting Beneficiation of Gemstones in Pakistan: A Mixed-methods Value Chain Analysis of Mining and Trade in Gemstone Industry of Khyber Pakhtunkhwa & Gilgit-Baltistan

Dr. Arshad Khan Bangash, Department of Sociology and Political Science, Bacha Khan University Charsadda

Collaborators:
Dr. Syed Owais, Department of Sociology, University of Peshawar

 

 

To map existing value stream from mining (at Swat and Skardu)

 

To compare Pakistan's mining and trade practices with gemstone industry giants such as India, China, Thailand, and Sri Lanka and draw lessons

 

To suggest concrete pathways and strategies for value addition in the mining, processing, and trade of gemstones in Pakistan at par with international competitive market.

 Data collection from KP and GB and is currently under analysis.  The initial findings disclosed that most of the precious stones is exported in rough form that affect economic profitability of the sector negatively. Also, there are long standing leasing issues in the region (Shamazoo, Fizagath and Shangla) that need to be addressed.

Further findings will identify gaps and trends of current mining and trade in Pakistan. 

 

 

07-LCF 84

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Bioconversion of hazardous Parthenium into nutrients enriched azolla and rock phosphate blended organic fertilizer

PI:
Dr. Fazli Wahid

, Department of Agriculture, University of Swabi

To transfer different biodegradable organic waste materials through value added bioagents into a standard vermicompost

 

To assess the role of high quality vermicompost on vegetables productivity and soil health as a possible substitute to chemical fertilizers

Preparation of standard vermicompost is established through different treatments of rock phosphate and other earthworms and PGPRs.

The product is in final stages and after checking data parameters of its mineral composition i.e. P, N, OM and pH will be produced and tested.

It is hoped that the ability of vermicompost application for vegetables production under greenhouse environment will substitute chemical fertilizers.

 

08-LCF-278

Research TitleResearch TeamResearch Problem/IssueSolution / Results
Integrated Conductive Polymers-Coated Sulfur Enriched Porous Carbons with Single-Walled Carbon Nanotubes as Self-Supported and Flexible Cathodes for Lithium-Sulfur Batteries

PI:
Dr. Adnan, Assistant Professor, Institute of Chemical Sciences, University of Swat

Co-PI:,
Dr. Mohammad Sohail, Assistant Professor, Institute of Chemical Sciences, University of Swat

To improve performance of Sulphur and Lithium batteries.

Sulfur cathode exhibited low intrinsic electronic conductivity.

Formation of the dissoluble intermediate's lithium polysulfides leads to the low coulombic efficiency, fast capacity fading and poor cycling performance. Materials used in conventional Li-S batteries are also non-bendable and corrode quickly.

Project fabricated the flexible electrodes that confine the movement of the dissoluble polysulfides by their encapsulation in phosphorous doped carbon microspheres (PCMs) and coated with the stable skeleton of the conducting polymers such as thiophene and pyrrole.

 

The low electronic conductivity problem of the sulfur electrode was solved with the addition of the SWCNTs framework and conducting polymers.

 

Conventional Li-S cathode materials was replaced with flexible Single-Walled Carbon Nanotubes and developed a novel hybrid free-standing electrode system that combines unique structure with good conductivity and high capacity.


​​

New Outcomes - FEB 2024

09-LCF-05 ( Approved Cost: Rs. 39,071,428 )

Research Title
Designing next generation high energy density Li-based batteries and advanced supercapacitors
​​Research Team
PI: Dr. Zahid Ali Ghazi
PIO: University of Peshawar
Total HR Engaged: 19 Male: 15, Female: 4
MS/PhDs Engaged/Produced: Male: 14, female: 3
Others: Male: 2, female: 5
​Research Problem/IssueThe primary focus of this project was to discover new materials and methodologies for the development of long life and stable Li batteries and supercapacitors. In addition, the development of energy storage/conversion laboratory for future research, the production of skilled and trained human resource and researchers and making linkages with industries for future large-scale applications and commercialization of Li-batteries and supercapacitors in Pakistan were also the goals of this project.
The overall, impact of this project is very significant. Lithium-ion batteries and supercapacitors have emerged as one of the main energy storage solutions in modern society. The marketing shares of batteries and capacitors have been rapidly increasing and showing a steady rising trend and expected to reach over USD 69 billion in next few years, which is growing at a substantial annual growth rate of over 16%. Furthermore, the huge demand of electric vehicles pushes further for the growth of battery/capacitor market.  The industry of  electric  vehicles (xEVs),  including  both  full  battery  electric  vehicles  (BEVs),  hybrid  electric  vehicles  (HEVs)  as well as  hybrid plug-in electric vehicles (HPEVs), are strongly committed to the mission of renewable, clean, and zero-emission.
Solution/Results
1. Developed Li-ion batteries/supercapacitors in the lab aiming the possible future commercialization in Pakistan
2. Prepared various  nanomaterials and nanocomposites for enhancing  the stability and energy density of Li batteries and supercapacitors
3. Developed energy storage and conversion laboratory for future research
Scientific/conference Paper/ Journals publications
10 Journal papers, 1 patent
Mass Media Dissemination (events, awards)
7 events, 1 award
Industrial partnership
None



10-LCF-07 ( Approved Cost: Rs. 27,869,554 )

Research Title
Organic and Inorganic Hybrid Materials for Energy Conversion and Storage
​​Research Team
PI: Dr. Raja Shahid Ashraf
PIO: Government College University, Lahore, Punjab
Total HR Engaged: 20 (Male: 06, Female: 14)
MS/PhDs Engaged/Produced: Male: 9, female: 26
Others: Male: 3, female: 26
​Research Problem/IssueThis project addressed the global challenge of meeting energy demands while mitigating environmental consequences, with a particular focus on Pakistan's struggle to balance energy, environment, and economic factors. The country heavily relies on fossil fuels, leading to economic burdens and environmental issues. The project aimed to design and produce innovative materials for hydrogen production and storage, successfully promoting the adoption of renewable technologies. Green hydrogen produced through this technology was envisioned to transform industries like fertilizers, steel, oil refining, and transportation. The collaborative research team, comprising experts from Pakistani and foreign universities, leveraged their collective skills to contribute to sustainable development, aligning with Pakistan's Energy Vision 2025 and global Sustainable Development Goals by 2030.
Solution/Results1. Preparation of nanocomposites based electrocatalysts
2. Preparation of hybrid porous materials for hydrogen storage
3. Characterization for structural confirmation of materials
4. Morphological studies (size, shape, crystal structure, surface defects, etc.) of materials
5. Optoelectronic analysis of materials
6. Hydrogen production and storage application
7. Modifications, scale-up synthesis and stability studies
8. Prototype production and device stability studies
Scientific/conference Paper/ Journals publications
6 Journal papers, 1 article,  10 conference papers
Mass Media Dissemination (events, awards)
​6 events, 1 poster award​
Industrial partnership
None

11-LCF-63 ( Approved Cost: Rs. 53,030,271 )

Research Title
Machine Learning Applications in Groundwater Resources and Management
​​Research Team
PI: Dr. Jan Muhammad
PIO: Balochistan University of Information Technology Engineering and management Sciences
Total HR Engaged: 28 (Male: 18, Female: 7)
MS/PhDs Engaged/Produced: Male: 13, Female: 5
​Research Problem/IssueThis project addresses the water scarcity in the study area, i.e. Quetta District, which has negatively affected the social and economic conditions of the local population. Groundwater, one of the main sources of water, has been overexploited. Some of the adverse socio-economic impacts of groundwater depletion are discussed in the following sections.
Water scarcity has caused widespread migration of people from rural areas to urban centers. Mass migration has a large population of Farmers who used to own farmland now work in other farmers' fields for daily wages. It is estimated that 30% of the population have migrated from their villages to other areas with adequate water and livelihoods. Other consequences of migration include increased job competition, which puts pressure on the labor market and creates conflicts between natives and immigrants.
Although all are involved in groundwater extraction, they accuse others of reducing the capacity of their tube wells. Drilling a new tubular well always comes with conflict in the community. Creating tension and anguish that disrupts the normal life of society. Given current trends in groundwater depletion, water scarcity can cause serious intergenerational problems. Proposed actions include planned tube well installations, monitoring water abstraction via meters, enforcing groundwater recharge methods, and mass awareness-raising through electronic, print, and social media to conserve and conserve water resources for future generations.
Solution/Results1. Determined the groundwater status and potential (water tables) for domestic, industrial, and agricultural purposes (based on past 2 decades' data through a resistivity survey).
2. Determined the sustainable yield and establish pumping thresholds of the groundwater table in the study area.
3. Designed and establish a smart monitoring network (sensors-based data loggers).
4. Proposed adaptive water management strategies/policies related to drought and wet periods that have a socio-economic impact on the local communities.
Scientific/conference Paper/ Journals publications
9 Journal papers, 9 conference papers
Mass Media Dissemination (events, awards)
13 events, 9 participations
Industrial partnership
1


12-LCF-69 ( Approved Cost: Rs. 20,433,649 )

Research Title
Coloration of polyester fabric at room temperature using advanced structure color technique: A practical approach towards green environment and energy saving
​​Research Team
PI: Dr. Ayaz Ali Memon
PIO: University of Sindh Jamshoro, Pakistan
Total HR Engaged: 5 Male: 5
MS/PhDs Engaged/Produced: Male: 2
Others: Male: 3
​Research Problem/IssueIn the textile apparel industry, color is the most fundamental feature that makes products appealing, beautiful and attracts consumers. Currently, almost all textile industry is using synthetic dyes for coloration which are toxic for environment. After coloration around 10% dye is wasted and mostly drained into the open environment, which ultimate becomes one of the main causes for polluting water. One method to solve this problem is to change the way coloration is done without using toxic dyeing process. Apart from toxicity, dyeing polyester fibers is achieved at high temperature which consumes huge amount of energy consumption. For example, temperature of 200 oC is required for continuous dyeing and 140 oC temperature is required in exhaust dyeing method for 1 hour. The Pakistan textile industry which is major part of exports around 50% is affected due to energy crises since 2010.
The technology of dyeing in textile industry has some drawbacks, such as high pollution, and high energy consumption. An idea that coloration of textile goods especially polyester fabrics based on structural color technique is to be introduced for textile industry in Pakistan.
Currently, the structural color is emerging technology that challenges the scientists to discover new trade of coloration while observing complex interactions between light and the sophisticated nanostructures existed in the natural world. The coloration from structure color technique is environment friendly because structural color is fadeless and no energy is lost from the color mechanism. Sliver nanoparticles (AgNPs) contain unique localized surface plasmon resonance (LSPR), by which AgNPs can produce brilliant physical colors. The physical color can be easily achieved by reducing the size in nanoscale and obtained regular shape of AgNPs. The physical color obtained from anisotropic AgNPs provides an alternate solution for the safe textile coloration in green method. AgNPs can not only be used to dye textile fibers/fabrics with variety of beautiful colors but can also have added advantage of antibacterial property.
Recently several surface treatments have been introduced which can generate cationic or anionic charge on polyester fabrics. These functional properties on polyester surface can enable to attract AgNPs and make polyester fabric colorful. Hence, our proposed method will be new way of coloration which promotes green environment methods as well as dyeing achieved at room temperature which can reduce the energy consumption in textile industry.
Pakistan is facing energy shortage and several environment issues including access to clean water. According to the International Monetary Fund (IMF), Pakistan ranked third among the countries facing severe water shortage. In May 2018, the Pakistan Council of Research in Water Resources (PCRWR) announced that by 2025, there will be very little or no clean water available in the country (Shukla 2018)
Our proposed project will contribute towards tow SDGs Goals, namely: 1. clean water and Sanitation and 2. Industry innovation and infrastructure by using non-toxic, green methods for dyeing and using innovative method that allows dyeing process at room temperature.
Solution/Results1. Synthesis and optimizing the physical colors from Metal NPs
2. Surface modification of polyester fabric
3. Dyeing of polyester fabric at room temperature using exhaust dyeing method
Scientific/conference Paper/ Journals publications
4 Journal/articles, 1 conference papers
Mass Media Dissemination (events, awards)
1 event
Industrial partnership
None


13-LCF-289 ( Approved Cost: Rs. 36,351,500 )

Research Title
Implementing community-based rural homestay tourism in Pakistan
​​Research Team
PI: Dr. Bilal
PIO: Hazara University Mansehra
Total HR Engaged: 20 (Male: 13, Female: 7)
MS/PhDs Engaged/Produced: Male: 12, Female: 9
Others: Male: 6, female: 5
​Research Problem/IssueThe purpose of this project was to plan, develop, and implement rural homestay tourism initiative in Mansehra. The project started with community mobilization in which more than 160 villages were targeted. Local villagers were contacted to make them aware of the costs and benefits of homestay tourism. A total of 253 households were identified who were willing to participate in homestay tourism initiative and who had fulfilled the criteria set for the homestay tourism business. We also initiated capacity building in terms of skills and knowledge at the level of the individual and the community. The local community in these villages currently lacks adequate training, education, awareness, and qualifications to face the challenge of constructing good quality homestay tourism. 92 capacity building sessions were held to educate locals about homestay tourism management.
We intend to identify the factors driving and inhibiting the management of the homestay by the community in villages of Mansehra, Balakot, and Kaghan.
Solution/Results1. Mobilization of local villagers and community to engage in homestay tourism. Making the community aware of the costs and benefits of homestay tourism.
2. Development of a comprehensive community-based homestay tourism framework (it is non-existent in Pakistan) by involving all stakeholders (e.g., local community, local government, KPK tourism authority, tourists, ministry of tourism).
3. Understanding of resident perceptions, values, and priorities regarding homestay tourism’s role in the community.
4. Capacity building of the local villagers in terms of homestay tourism management and general business management skills.
5. Market rural homestay tourism to attract tourists to visit homestays.
6. Investigating tourists’ feedback, improving the homestay tourism model, and disseminating the findings to the stakeholders. 
Scientific/conference Paper/ Journals publications
1 conference paper
Mass Media Dissemination (events, awards)
None
Industrial partnership
None


14-LCF-306 ( Approved Cost: Rs. 18,837,000 )

Research Title
Local manufacturing of industrial important enzymes (lipase, laccase and xylanase) using cell free extract approach and microbial fermentation technology
​​Research Team
PI: Dr. Haneef Ur Rehman
PIO: University of Turbat
Total HR Engaged: 3 (Male: 2, Female: 1)
MS/PhDs Engaged/Produced: Male: 1, Female: 1
​Research Problem/IssueThe project was based on uses of enzymes in different industrial applications are increasing globally. These enzymes can be used in different industries including food industries, detergent industries, cosmetic industries, pharmaceutical industries, wastewater treatment, leather industries as well as biofuel production and diagnosis. A semi-automated bioreactor will be designed using agriculture and vegetable wastes as substrates through microbial fermentation technology. The project allows us to develop cost effective and economically feasible protocols for manufacturing and distribution of lipase, laccase and xylanase enzymes in Pakistan. The aim of project to harness synthetic biology techniques combined with flow bioreactor system of off-patent affinity tags which bind to particular substrates for column or well-based protein purification. The motivation for the project is to make more affordable enzymes for food, detergent and textile companies.
The proposed project has been conducted to develop local manufacturing set-up to produce lipase, xylanase and laccase. The scope of the project is to develop biotechnological business opportunity in Pakistan through commercial production of industrial important enzymes. The proposed project will boost the enzymes productivity and earn market space in multiple industries such as food and beverages, which include dairy, bakery, poultry, meat and fruit juices.
Solution/Results1. Isolation and Identification of lipases, laccase and xylanase producing microbial strains.
2. Characterization and kinetic studies of lipases, laccase and xylanase enzymes
3. Screening of agriculture and vegetable wastes as cheap source of substrate for enzyme production.
4. To optimize the commercial scale production of lipase, laccase and xylanase enzymes.
5. Synthetic biology techniques with automation to undertake rapid combinatorial flow reactor with purification set up which bind to particular substrates for column or well-based protein purification 
Scientific/conference Paper/ Journals publications
1 Journal
Mass Media Dissemination (events, awards)
None
Industrial partnership
None


15-LCF-349 ( Approved Cost: Rs. 23,332,350 )

Research Title
Development of multipurpose Synthetic Microbiome Based
Transplants (SMT) to recover Gut Microbiome Dysbiosis
​​Research Team
PI: Dr. Raees Khan
PIO: National University of Medical Sciences, Rawalpindi
Total HR Engaged: 15 (Male: 11, Female: 4)
MS/PhDs Engaged/Produced: Male: 3
Others: Male: 6, Female: 4
​Research Problem/IssueFecal microbiota transplantation (FMT) has recently been implied as a promising treatment option in various clinical conditions. Currently, more than 300 clinical trials are going on assessing the significance of FMT in a variety of health conditions. Such huge number of trials for a single therapy type against different health conditions make it a leading therapy type outcompeting entire other drugs under evaluation. However, conventional FMT has a variety of limitations that make such therapies problematic and lead to transient results. These limitations include (i) FMT mediated transfer of pathogenic microorganisms from donors to recipients12, (ii) Lack of reproducibility11, (iii) Lack of stability resulting in transient outcomes13 and (iv) high donor screening costs. Therefore, there is an utmost need to develop a safe, stable, sustainable and economical alternative to conventional FMT. Additionally, FMT has never been utilized in Pakistan, and there is no single case/trial going on locally to assess its significance.
This project aims to develop multipurpose, safe, stable, sustainable and economical synthetic microbiome-based transplants (SMT) as an alternative to conventional FMT for the quick recovery of gut microbiome dysbiosis and to ultimately use this SMT for the treatment of various gut inflammatory disorders locally in future. The proposed SMT can be utilized in treating a variety of health conditions including CDI, UC, IBD, UC, and CD locally, as these health conditions are a serious concern in Pakistan. However, to test the efficacy of SMT, initially it will be tested for the quick and efficient recovery of disturbed gut microbiota post antibiotic administration in animal model. Additionally, the impact of SMT in minimizing the enrichment and selection of antibiotic resistant organisms in the gut and their dissemination to the environment will also be assessed. Once the safety and efficacy of SMT in animal model is confirmed, SMT based transplants will then be used in humans in future to treat a variety of health conditions including CDI, CD, IBD, IBS, UC, which is the ultimate goal of this study. Additionally, SMT has the potential to be utilized in a variety of health conditions other than gut inflammatory disorders as well. Thus overall, the utilization of SMT can significantly improve public health locally and globally.
Additionally, the proposed SMT strategy could be a novel far better alternative to conventionally used probiotics as well. Both the FMT and probiotics currently hold a market value of 40 billion USD.
Solution/Results1. To develop a safe, sustainable multipurpose synthetic microbiome-based transplants (SMT) as an alternative to conventional FMT.
2. To utilize SMT approach in future in the treatment of dysbiosis mediated health conditions including CDI, CD, IBD, IBS and other inflammatory disorders of the gut. All these mentioned health conditions are of major concerns locally.
3. To utilize SMT in minimizing the negative health outcomes associated with antibiotics mediated disruption of the gut microbiome.
4. Establishing the setup and fulfilling the initial requirements necessary for conducting this research.
5. Selection of healthy human donors
6. Preparation of fecal samples from healthy human donors and processing for microbiome separation, metagenomic DNA extraction, RABs isolation and other important bacterial cultures isolation and identification.
7. Construction of synthetic microbiome-based transplants (SMT) utilizing RABs and other important taxa of human fecal microbiome.
8. Transplantation of SMT into mice with disturbed gut microbiome.
9. Testing the efficacy of SMT in the recovery of gut microbiome dysbiosis and as probiotics.
10. Comparative microbiome analysis of test and control groups to see how SMT affect the recovery and structure of gut microbiome.
11. Comparative shotgun metagenome analysis of test and control groups to see how SMT affect the functional capabilities and profile of the gut microbiome.
12. SCFA analysis, assessment of pro-inflammatory genes expression and histopathological analysis will further shed light on how SMT pose protective/probiotic effect on host and host gut microbiome.
13. Translation of the research results in light of the data produced from the project and use of that data for further development and enhancement of SMT for future clinical trials in human and for product commercialization.
Scientific/conference Paper/ Journals publications
1 Journal
Mass Media Dissemination (events, awards)
1 event
Industrial partnership
None


16-LCF-365 ( Approved Cost: Rs. 23,253,000 )

Research Title
Efficacy of the Poverty Graduation Model and its Impacts on Socio Economic Wellbeing and Governance Mainstreaming of Poor Youth
​​Research TeamPI: Dr. Nasir Iqbal
PIO: COMSATS University Islamabad
Total HR Engaged: 62 (Male: 20, Female: 42)
MS/PhDs Engaged/Produced: Male: 2, female: 3
​Research Problem/IssueA core challenge for policymakers is to sustain poverty reduction through strategies of uplift and prevention, reflected in the evolving programs for holistic social protection. This project addresses a central shortcoming of mainstream approaches to poverty reduction. The ‘poverty graduation’ approach of many poverty reduction programs can be problematic if it approaches poverty as a binary state. This binary approach can critically overlook the dynamic experiences of poverty, and how the prevalence and penetration of vulnerability and insecurity structure the daily lives of the poor. We believe that to further determine the effectiveness of poverty graduation programs, understanding the factors which strengthen household resilience against adverse shocks and hazards is critically and urgently necessary to design and implement smart poverty graduation programs.
We aim to generate rich insights into the ways social protection programs and poverty graduation models can be made more accurate and effective in holistically reducing poverty. This project will therefore explore the necessary conditions for transformative resilience, which is achieved when households can cope with shocks, maintain their income sustainably above the poverty line, and still make steady livelihood progress.
Solution/Results1. Project evaluates the effectiveness of various poverty gradation interventions under National Poverty Graduation Program. These interventions include
a.       Transfer of Tangible Asset with supplementary Enterprise Development    Training, to Poor and Ultra Poor Households
b.        Transfer of Tangible Asset with Interest-Free Loans (received by beneficiaries a few months after the transfer of tangible assets), to Poor and Ultra Poor Households
c.        Membership of NPGP Beneficiaries, in Processing-Side, New Generation Business Cooperatives
2. To examine how the macro policy framework of Pakistan affects poverty dynamics and generate recommendations to improve the existing policies and programs in light of study findings, and if required, suggest new ones which can contribute to poverty reduction
3.  Keeping in view the ongoing Covid-19 pandemic and the increasing climatic shocks, to suggest a shock responsive.
Scientific/conference Paper/ Journals publications
1 Journal
Mass Media Dissemination (events, awards)
1 event, 1 policy
Industrial partnership
None


17-LCF-415 ( Approved Cost: Rs. 8,156,750 )

Research Title
Development of consortium for enhanced recovery of residual petroleum: Step towards sustainable energy exploration and exploitation
​​Research Team
PI: Dr. Arshia Butt
PIO:  Capital University of Science and Technology, Islamabad
Total HR Engaged: 5 (Male: 3, Female: 2)
MS/PhDs Engaged/Produced: Male: 4, female: 8
Others: Female: 1
​Research Problem/IssueThe global oil output is declining due to rising population and the need for energy. Microbial-enhanced oil recovery (MEOR) is a technique that uses organisms and their metabolites to boost oil recovery from reservoirs. MEOR increases utility and
decreases capillary pressures, keeping oil in the reservoir. It offers low energy requirements and independence from changes in crude oil prices. MEOR also breaks down complex organic compounds, making crude oil more mobile. The study
focuses on the Isolation, purification, screening, and characterization of thermotolerant aerobic and anaerobic bacterial flora at varying growth conditions.
The purpose of study is to recognize the genetic diversity of bacterial strains using a polyphasic taxonomic approach and develop a consortium with functional characterization and prototype. This approach could help address the declining oil output and energy need in various regions.
Solution/Results1. Isolated and purified thermo-tolerant and heavy metal tolerant anaerobic, and aerobic bacterial strains by using a variety of substrates and growth conditions.
2. Identified nonculturable microbial diversity of selective sites for total microbial profiling.
3. Estimated functional profile of sites by analyzing the abundance of nitrogen and sulphur reducing genes.
4. To estimate the surfactant properties of purified bacterial strains to check  their role in reducing surface tension of oil water  phase.
5. To develop and functionally characterize various combinations of strains to design a consortium for efficient biotransformation.
6. To develop and test a bio stimulant injection prototype containing designed consortium for pilot scale application.
Scientific/conference Paper/ Journals publications
1 conference paper
Mass Media Dissemination (events, awards)
1 event
Industrial partnership
1 partnership


18-LCF-418 ( Approved Cost: Rs. 21,472,950 )

Research Title
Development of climate smart cotton by editing Arginase (GhArg) genes using genome editing (CRISPR/Cas) tools
​​Research Team
PI: Dr. Haneef Ur Rehman
PIO: University of Turbat
Total HR Engaged: 3 (Male: 2, Female: 1)
MS/PhDs Engaged/Produced: Male: 1, Female: 1
​Research Problem/IssueGlobal warming and environmental changes continue, traditional cotton breeding techniques must be supplemented with new molecular tools so that cotton remains competitive against synthetic fiber. Although transgenic technology has enabled significant progress on breeding new cultivars with useful agronomic traits, the challenge remains for cotton to be more tolerant to numerous environmental extremes.
The post-genomic era confronted researchers with the need to develop efficient tools for gene function studies and diverse approaches have been developed to accomplish target specific genome editing, such as CRISPR-Cas systems. CRISPR/Cas9 has been rapidly adopted as the preferred genome editing tools and has been widely used in several plants including cotton plants.
Recent studies demonstrated that arginine (ARG) is the precursor of nitric oxide (NO) in roots catalyzed by nitric oxide synthase (NOS), and NO plays a key role in the lateral root formation. In Arabidopsis reduced activity of arginase may increase NO contents in roots and therefore improved the lateral roots in transgenic plants. Our preliminary research showed that there are two, highly similar, orthologous, cotton arginase genes (GhARG), Gh_A05G2143 and Gh_D05G2397, in the A and D chromosomes. CRISPR/Cas system may be helpful in improving lateral root system for abiotic stress tolerance and also ensuing adaptation of cotton on a variety of soils.
The growth of cotton plants is strongly influenced by several abiotic stresses such as drought and high temperature. Therefore, strengthening drought and high temperature tolerance mechanisms through improvement of root system architecture (RSA) is essential for modern varieties. It is anticipated that the project will result in the development of stress-tolerant cotton cultivars for sustainable cotton production by using gene editing approaches such as CRISPR-Cas toolkit.
This project focuses on crop plant improvement. Because cotton is the backbone of country, this project specially focuses on the restoration of cotton in the country.
Solution/Results​1. Selection of suitable Plant material (commercial cotton variety with regeneration potential)
2. Bioinformatic analysis, design and synthesis of Grna
3. Cloning of three gRNAs into plant expression vector and molecular confirmation through PCR and restriction digestion analysis
4. Cotton transformation, selection and regeneration of transformants using established protocols
5. Screening of modified regenerated cotton plants using molecular and biochemical techniques including DNA sequencing in T0 generation
6. Glasshouse or small-scale field trials for seed increase for further generations​
Scientific/conference Paper/ Journals publications
1 conference paper
Mass Media Dissemination (events, awards)
None
Industrial partnership
1 partnership